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14-749M TAO1 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.

TAO1 Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-749M
250 µg  Also available in 10 μg size (cat#14-749) and in bulk (cat# 14-749-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-749M
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTAO1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327.
References
Product Information
Presentation10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
PurityPurity 93% by SDS PAGE and Coomassie blue staining
SourceExpressed by baculovirus in Sf21 cells.
Specific Activity1179 U/mg, where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
Entrez Gene Number
Gene Symbol
  • TAOK1
  • MAP3K16
  • KIAA1361
  • PSK2
  • MARKK
  • FLJ14314
  • hKFC-B
Protein TargetTAO1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q7L7X3 # Phosphorylates MKK3 (By similarity). Activates the p38 MAP kinase pathway through the specific activation of the upstream MKK3 kinase.
SIZE: 1001 amino acids; 116070 Da
SUBUNIT: Self-associates. Interacts with MKK3 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q7L7X3 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.
Molecular Weight41 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-749) and in bulk (cat# 14-749-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-749M 04053252582455

Productos y aplicaciones relacionados

Alternative Packsize

Número de referencia Descripción
14-749 TAO1 Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases