SpheriTide™ – the sustainable support for peptide synthesis

SpheriTide™ resin is a novel hydrophilic, high-load support for both research and large-scale production of peptides. It consists of poly-ε-lysine cross-linked with sebacic acid.

Features

• Loading of base polymer is approximately 3 mmol/g
  
• Loading of HMPA SpheriTide™ resin is typical 1.8 - 2.2 mmol/g, of Rink Amide SpheriTide™ 1.0 - 1.3 mmol/g
  
• Low bed-volume to substitution ratio, thus less solvent waste and lower excesses of reagents
  
• Ideal for research and large scale batch synthesis
  
• Compatible with a wide range of polar and nonpolar solvents, including MeOH, water, DCM, DMF
  
• Biodegradable – polymer is completely degraded by proteolysis
  
• Starting materials for resin derived from renewable resources

Sustainable support

In contrast to other supports that are manufactured from products derived from fossil fuels, HMPA SpheriTide™ and Rink Amide SpheriTide™ starting materials are obtained from renewable biological sources. Poly-ε-lysine is a naturally occurring short-chain polyamide consisting of 25-35 lysine residues linked through their α-carboxyl and ε-amino groups. It is produced by bacterial fermentation for use as a food preservative whereas sebacic acid is made from castor oil for use in the textile industry. SpheriTide™ is thus one of the first sustainable supports especially designed for peptide synthesis - it is also biodegradable as the polyamide backbone is susceptible to proteolysis.

Great swelling properties, high functionality levels

Because SpheriTide™ resin is essentially just a polymer of lysine, very high levels of functionality can be obtained without comprising its swelling and usability in peptide synthesis. Typically, the unloaded polymer has 3 mmol/g of reactive amino groups – this is approximately 2 times that of hydrophobic polystyrene resins and 3-10 times that of other comparable hydrophilic supports such as NovaPEG, PEGA and NovaSyn® TG. Due to addition of the mass of linker, loadings of HMPA SpheriTide™ resin and Rink Amide SpheriTide™ resin drop to 1.8 - 2.2 mmol/g and 1.0 - 1.3 mmol/g, respectively, which is still significantly higher than Wang and Rink Amide AM resins. This high level of substitution means bed-volumes can be kept to a minimum. This results in lower solvent consumption and allows the use of lower excesses of activated amino acids since more concentrated solutions can be employed.