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03-0156-00 SMC™ Human IL-13 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-13 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-13 in human serum and

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Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0156-00
96 well assay  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Species ReactivityKey Applications
NhP, R, Ca, GpSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0156-00
Trade Name
  • SMC®
DescriptionSMC™ Human IL-13 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-13 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 13 (IL-13) is secreted by a number of cells including T helper cells, CD4 cells and a various white blood cells and is thought to be a mediator of allergic inflammation in many tissues via the induction of IgE synthesis by B cells. Although primarily associated with the induction of airway disease, IL-13 has also been shown to inhibit the production of proinflammatory cytokines by monocytes and macrophages suggesting it has important anti-inflammatory properties.
Product Information
Components
  • Assay Buffer
  • Human IL-13 Beads
  • Standard Diluent
  • Human IL-13 Detection Antibody
  • Human IL-13 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=5): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-13 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-13 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-13 v2 kit, #03-0109-02:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity
  • Non-human Primate
  • Rat
  • Canine
  • Guinea Pig
Species Reactivity NoteAlthough IL-13 was detectable in non-human primate, rat, canine, and guinea pig samples, the assay has not been validated for these species.
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 90% with a range of 86-102% and 5 plasma samples spiked with average recovery of 92% with a range of 87-96%.
Physicochemical Information
Sensitivity
  • LLOQ range: 0.02-0.08 pg/mL, refer to CoA for lot-specific LLOQ
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size96 well assay
Global Trade Item Number
Número de referencia GTIN
03-0156-00 04054839544101