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03-0146-00 SMC™ Amyloid Beta 1-42 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Amyloid Beta 1-42 (Aβ42) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Aβ42 in hum

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Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience, Metabolism Research Sub-Category: Inflammation & Autoimmune Mechanisms, Metabolism, Obesity
03-0146-00
96 well assay  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0146-00
Trade Name
  • SMC®
DescriptionSMC™ Amyloid Beta 1-42 High Sensitivity Immunoassay Kit
OverviewThe SMC™ Amyloid Beta 1-42 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationAmyloid Beta refers to the short peptides that are released into circulation following the proteolysis of the transmembrane protein amyloid precursor protein (APP), whose function is unclear but thought to be involved in neuronal development and function. The most common peptides generated from APP cleavage are Aβ1-40 and Aβ1-42, with the latter isoform implicated in the formation of toxic oligomers, fibrils, and plaques in the diseased brain. Reduced levels of soluble Aβ1-42 in the cerebrospinal fluid (CSF) are associated with Alzheimer’s disease (AD), leading to the adoption of this peptide as an AD biomarker.
Product Information
Components
  • SMC™ Amyloid Beta Assay Buffer
  • SMC™ Amyloid Beta 1-42 Coated Beads
  • SMC™ Amyloid Beta Standard Diluent
  • SMC™ Amyloid Beta Detection Antibody
  • SMC™ Amyloid Beta 1-42 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <10%
  • Inter-assay CV% average (n=6): <10%
Quality LevelMQ200
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Amyloid Beta 1-42 (Aβ42) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Aβ42 in human, mouse and rat cerebrospinal fluid (CSF) and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Cross ReactivityNo significant cross-reactivity to Aβ38 and Aβ40 was observed.
Accuracy
  • Minimum of 6 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.98 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size96 well assay
Global Trade Item Number
Número de referencia GTIN
03-0146-00 04054839166433