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23-023M SCF-Skp2 Protein Complex, Active, 250 µg

Active complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

SCF-Skp2 Protein Complex, Active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Enzyme Assays
23-023M
250 µg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E3
Description
Catalogue Number23-023M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSCF-Skp2 Protein Complex, Active, 250 µg
OverviewAXIS™ Axon Isolation Devices, 450 µm long channels, 5 devices
Alternate Names
  • Skp2/Cks1 complex
  • SCFSkp2
Background InformationThe SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition. Complex formation of SCFSkp2 with Cks1 is required for the degradation of p27. An increased level of Skp2 and reduced level of p27 is linked to poor prognosis in a number of cancers
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of five (5) full length, recombinant human enzymes: Skp1, Skp2, Cul1, Rbx1 & Cks1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Cascade ComponentE3
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size250 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
23-023M 04053252294396

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Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E3 Ligases
Life Science Research > Proteins and Enzymes > Ubiquitin E3 Ligases