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HTS060RTA Ready-to-Assay™ V2 Vasopressin Receptor Frozen Cells

Human V2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Host Cell: Chem-1 Entrez Gene Number: NM_000054
HTS060RTA
1 kit  
Purchase on Sigma-Aldrich

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Tabla espec. clave

SpeciesHost CellsProtein Target
Human Chem-1V2
Description
Catalogue NumberHTS060RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ V2 Vasopressin Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V1A, V1B and V2, are GPCRs; V1A and V1B couple to Gq and calcium release, whereas V2 couples to Gs. V2 expressed in renal collecting ducts plays an important role in regulating renal free water excretion (Birnbaumer, 2000). Mutations in V2 result in X-linked nephrogenic diabetes insipidus, a syndrome in which the kidney is unable to concentrate urine, leading to dehydration and hypernatremia (Birnbaumer, 2001). Conversely, elevated levels of AVP lead to hyponatremia in the syndrome of inappropriate antidiuretic hormone secretion (SIADH), congestive heart failure or cirrhosis, and V2 selective antagonists have been developed to treat these conditions (Verbalis, 2002). Chemicon’s cloned human V2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant V2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between V2 and its ligands.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman V2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetV2
Target Sub-FamilyVasopressin
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HTS060RTA 04053252527630

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Categorías

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells