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HTS144RTA Ready-to-Assay™ NK2 Tachykinin Receptor Frozen Cells

Human NK2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_001057
HTS144RTA
1 kit  
Purchase on Sigma-Aldrich

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Descripción

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Tabla espec. clave

SpeciesHost CellsProtein Target
Human Chem-1NK2
Description
Catalogue NumberHTS144RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ NK2 Tachykinin Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The tachykinin peptide family in mammals comprises three peptides, substance P, neurokinin A and neurokinin B, which bind to the tachykinin receptor family of GPCRs, NK1, NK2 and NK3 (Severini et al., 2002). Tachykinins have prominent activity in the GI system, in which they stimulate intestinal contraction and salivation; these effects are mediated by NK1 and NK2. Experiments with selective antagonists of NK2, SR 48,968 and MEN 10,376 indicate that NK2 mediates neurokinin- induced constriction of pulmonary artery and bronchus (Maggi et al., 1993). NK2 antagonists display efficacy in treatment of irritable bowel disorder (Lecci et al., 2004). Millipore’s cloned human NK2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NK2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between NK2 and its ligands.
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman NK2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetNK2
Target Sub-FamilyTackykinin /neurokinin
Cell Line Type
  • GPCR Cell Lines
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size1 kit
Global Trade Item Number
Número de referencia GTIN
HTS144RTA 04053252555381