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71772 ProteoExtract® Transmembrane Protein Extraction Kit

71772
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
71772-3 20 rxn
Description
OverviewThe ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Catalogue Number71772
Brand Family Novagen®
References
ReferencesNakamura, E., et al. 2008. Cancer Sci. 99, 1390.
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 25-33

Toxic if swallowed.
Danger of cumulative effects.
S PhraseS: 37-45

Wear suitable gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
71772-3 04055977250909

Documentation

ProteoExtract® Transmembrane Protein Extraction Kit Certificados de análisis

CargoNúmero de lote
71772

Referencias bibliográficas

Visión general referencias
Nakamura, E., et al. 2008. Cancer Sci. 99, 1390.

Folleto

Cargo
Kit SourceBook - 2nd Edition EURO
Western Blotting Tools

Citas

Título
  • Waetzig G.H., et al. 2010. The Journal of Biological Chemistry 285, 1781.
  • Ernst, C.M., et al. 2009. doi: 10.1371/journal.ppat.1000660
  • Protocolos de usuario

    Cargo
    TB477