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524715 Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE

524715
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
524715-50UL Ampolla de plást. 50 ul
Description
OverviewRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).
Catalogue Number524715
Brand Family Calbiochem®
SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
References
ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
FormLiquid
FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
524715-50UL 04055977197655

Documentation

Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda Certificados de análisis

CargoNúmero de lote
524715

Referencias bibliográficas

Visión general referencias
Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-July-2007 JSW
SynonymsPDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
DescriptionRecombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.
FormLiquid
FormulationIn sodium phosphate buffer, 20% glycerol, pH 7.2.
Activity≥50,000 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.