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14-497M PKC η Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta. For use in Kinase Assays.

14-497M
250 µg  Also available in 10 μg size (cat#14-497) and in bulk (cat# 14-497-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-497M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKC η Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta.

PKC Lipid activator (Catalog # 20-133) is required for use.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PKC eta expressed in Sf21 cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
Gene Symbol
  • PRKCH
  • PRKCL
  • PKCL
  • MGC26269
  • nPKC-eta
  • MGC5363
  • PKC-L
Protein TargetPKCη
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P24723 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
SIZE: 682 amino acids; 77563 Da
TISSUE SPECIFICITY: Most abundant in lung, less in heart and skin.
DOMAIN: SwissProt: P24723 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
DISEASE: SwissProt: P24723 # Defects in PRKCH may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Molecular Weight78.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of PKC Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-497) and in bulk (cat# 14-497-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-497M 04053252276064

Documentation

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Número de referencia Descripción
14-497 PKC η Protein, active, 10 µg

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Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases