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14-483M PKCγ Protein, active, 250 µg

Active, recombinant, full-length human PKCγ, containing N-terminal His6 tag. For use in Kinase Assays.

PKCγ Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-483M
250 µg  Also available in 10 μg size (cat#14-483) and in bulk (cat# 14-483-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-483M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKCγ Protein, active, 250 µg
OverviewRecombinant, full-length human PKCγ, containing N-terminal His6 tag

PKC Lipid activator (Catalog # 20-133) is required for use.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, recombinant, full-length human PKCγ, containing N-terminal His6 tag. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PKCγ expressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
Gene Symbol
  • PRKCG
  • SCA14
  • MGC57564
  • PKC-gamma
  • PKCG
  • PKCC
Protein TargetPKCγ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05129 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 697 amino acids; 78448 Da
SUBUNIT: Interacts with CDCP1.
DISEASE: SwissProt: P05129 # Defects in PRKCG are the cause of spinocerebellar ataxia 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord.
SIMILARITY: SwissProt: P05129 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Molecular Weight79.5kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-483) and in bulk (cat# 14-483-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-483M 04053252625701

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Alternative Packsize

Número de referencia Descripción
14-483 PKCγ Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases