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14-604M-D PI3 kinase (p110δ/p85α)

Complex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.

PI3 kinase (p110δ/p85α): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-604M-D
250 μg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-604M-D
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPI3 kinase (p110δ/p85α)
OverviewComplex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system
References
Product Information
Presentation25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, and 50% glycerol
Quality LevelMQ100
Applications
ApplicationComplex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system, for use in enzyme kinetics, screening inhibitors, and selectivity profiling.
Key Applications
  • Kinase Assay
Biological Information
Purity≥75%
SourceSf9 insect cells
Specific Activity500 pmol/min/µg.
Entrez Gene Number
Entrez Gene SummaryPhosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha (MIM 171834), p110-beta (MIM 602925), and p110-gamma (MIM 601232). p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins (see MIM 171833) and with GTP-bound Ras.[supplied by OMIM]
Gene Symbol
  • PIK3CD
  • p110d
  • PI3K
  • p110D
  • p110delta
Target Sub-FamilyPI3K/PI4K
UniProt Number
UniProt SummarySIZE: 1044 amino acids; 119479 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS (By similarity).
TISSUE SPECIFICITY: Expressed predominantly in leukocytes.
PTM: Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
SIMILARITY: SwissProt: O00329 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.
Molecular Weight146 kDa (p110δ), 84 kDa (p85α)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Kinase assay: A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 uM ATP, 200 μM PI:PS substrate and p110δ/p85α for 17 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for > 6 month at –80°C from date of receipt.
Packaging Information
Material Size250 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-604M-D 04055977276633