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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PCR Cleanup Filter Plates

Remove primers and unincorporated dNTPs in one step Más

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Descripción

Especificaciones

Información para pedidos

Filter PlatesBorrar Clasificación y Filtrado Show Filter Hide Filter
Número de referencia	Descripción	Tamaño de envase
LSKMPCR10	Placa de filtración MultiScreen PCR_µ96	10
LSKMPCR50	Placa de filtración MultiScreen PCR_µ96	50
MSNU03010	Placa de filtración MultiScreen PCR₉₆	10
MSNU03050	Placa de filtración MultiScreen PCR₉₆	50
S384PCR10	Placa de filtración MultiScreen PCR₃₈₄	10
S384PCR50	Placa de filtración MultiScreen PCR₃₈₄	50

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Required EquimentBorrar Clasificación y Filtrado Show Filter Hide Filter
Número de referencia	Descripción	Tamaño de envase
MSVMHTS00	Colector de vacío MultiScreen_HTS	1
WP6111560	Bomba de servicio químico, 115 V/60 Hz	1
WP6122050	Bomba de servicio químico, 220 V/50 Hz	1
WP6110060	Bomba de servicio químico, 100 V/50–60 Hz	1

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Documentation

Referencias bibliográficas

Visión general referencias	Aplicación
PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D. BioTechniques 29:844-857 1999
A Concise Guide to cDNA Microarray Analysis P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000) 1999	PCR
A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309 J.Vet Diagn Invest 8:, 304-309 1996	Nucleic Acid Purification and Concentration
Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E. BioTechniques. 19: 2, 282 – 285 1994	Nucleic Acid Purification and Concentration

Preguntas frecuentes

Pregunta	Respuesta
My recoveries with the MultiScreen₃₈₄-PCR plate are lower than expected. How can I improve them?	Here a few things to check: Make sure the vacuum is set to 10"Hg. For detergent free PCR buffers, dilute sample up to 100ul prior to filtration. Make sure the product is filtered to dryness. This will take at least 15 minutes. Ensure adequate mixing by either automated pipetting or agitation on a shaker.
Will mineral oil be removed by the MultiScreen 384-PCR plate?	No. Oil will resuspend with PCR products. All 384 thermocyclers use "Hot Bonnet" technology, however, eliminating the need for oil.
When using the Multiscreen PCR plates, what is the minimum and maximum DNA loading capacity per well?	The minimum DNA loading capacity is one microgram per well. There is no upper limit.
What is the minimum recovery volume possible when using the MultiScreen-PCR plates?	The minimum recovery volume is 50µl. Use of smaller volumes can be used but will result in a decrease in DNA recovery.
What are the dimensions of the Multiscreen-PCR plate?	The plate length is 123.4mm. The plate width is 82.7mm. the plate depth is 14.6mm without the cover. 16.5mm with cover.
When using the Montage kits how can I tell when filtration in the MultiScreen plate is complete?	Filtration is complete when the individual wells of the MultiScreen plate are empty and the membranes appears shiny. Filtration usually takes about 10 minutes, depending on starting volume.
What is the DNA binding capacity of the MultiScreen FB for PCR purification?	The DNA binding capacity is 4-5ug per well.
When using the Montage PCR-96 kit, what is the recommended procedure for resuspension of PCR fragments post filtration?	Avoid manual pipetting. The use of a plate shaker or automated pipettor (on robotic liquid handling system) is highly recommended.
What is the minimum resuspension volume for product recovery from the Montage PCR96 Kit?	50 microliters per sample.
What is the maximum binding capacity for DNA in the PCR Product Purification protocol using the MultiScreen-FB plate?	The binding capacity of the MultiScreen FB, after treatment with "binding" buffer is 4-5ug of DNA per well.

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Categorías

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates

C7479Remove primers and unincorporated dNTPs in one step

Our PCR filter plates are fast, automatable solutions for high-throughput PCR purification. Available in 96- and 384-well formats and a µ96-well format for small volumes, MultiScreen® PCR plates use a size-exclusion membrane and vacuum filtration to provide a one-step protocol with excellent results. No centrifugation or precipitation is required. Full sample recovery from the top of the plate enhances compatibility with liquid handling systems. MultiScreen® PCR_µ96 and MultiScreen®PCR₃₈₄ filter plates provide high recovery on even the smallest miniaturized reactions. Working volumes <150 µL conserve reagents and save money. For volumes >150 µL, use MultiScreen®PCR₉₆ 96-well filter plate.

Features & Benefits

High purity and high recovery
No centrifugation or precipitation
Fast processing times, automation compatible
MultiScreen® PCR_µ96 filter plate recommended for small fragments (1-150 bp)

Protocol

1.	Load PCR reactions.
2.	Filter with Millipore vacuum manifold for 5–10 minutes or until wells are dry.
3.	Add water or buffer to each well. Agitate by shaking or pipetting. Retrieve purified samples by aspiration.

Selection Guidelines

Specifications
Product name	Reaction volume	Number of samples	Mode of operation	Recommended applications	Features
MultiScreen^® PCR_µ96 Filter Plate	20 – 150 µL	96	Vacuum	Sequencing Genotyping Microarray	• Highly concentrated final sample • Reaction miniaturization
MultiScreen^® PCR₉₆ Filter Plate	150–300 µL	96	Vacuum	Microarray	• Volumetric capacity
MultiScreen^® PCR₃₈₄ Filter Plate	20–100 µL	384	Vacuum	Sequencing Genotyping Microarray	• Highly concentrated final sample • Reaction miniaturization • Ultra-high throughput

Recovery

Product name	Recovery of 137 bp PCR fragment*	Recovery of 301 bp PCR fragment*	Recovery of 657 bp PCR fragment*	Recovery of 1159 bp PCR fragment*	% Primer removal (20 bases), 100 µL volume
MultiScreen^® PCR_µ96 Filter Plate	+	++	++	++	99.8%
MultiScreen^® PCR₉₆ Filter Plate	--	+	++	++	98.7%
MultiScreen^® PCR₃₈₄ Filter Plate	+	++	++	++	99.5%
-- Not Recommended
+ Good Recovery
++ Best Recovery
*Results will vary based on starting concentration, load, and buffers used. Data obtained using the following concentrations: 137 bp: 10 ng/µL 301 bp: 30 ng/µL 657 bp: 55 ng/µL 1159 bp: 71 ng/µL

Performance

Get high reproducibility with MultiScreen PCR₉₆ Filter Plate

Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR<sub>96</sub> filter plate.

Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR₉₆ filter plate.

Long Sequencing Reads

Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR<sub>96</sub> filter plate (Thermo Sequenase® II dye terminator).

Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR₉₆ filter plate (Thermo Sequenase® II dye terminator).

Suitable for Microarrays

Enlarged section of a 19,299 element microarray (image reproduced with permission from Dr. John Quakenbush, The Institute for Genomic Research). High quality microarrays can be manufactured using PCR products purified with MultiScreen PCR products. Reference: Hegde, P., R. Qi, K. Abernathy, C. Gay, S. Dhaarp, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee and J. Quakenbush. 2000. A concise guide to cDNA microarray analysis. BioTechniques 29: 548–562.

Applications

PCR Cleanup, Sequencing, Genotyping, Microarray, RNAi Purification, Microarray Purification, Genomic DNA Concentration

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