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14-584M PAK4 Protein, active, 250 µg

Active, N-terminal His6 & N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end, for use in Kinase Assays.

PAK4 Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-584M
250 µg  Also available in 10 μg size (cat#14-584) and in bulk (cat# 14-584-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-584M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPAK4 Protein, active, 250 µg
Overviewexpressed by baculovirus in Sf21 cells
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6 & N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal His6 and N-terminal GST-tagged, recombinant, human PAK4 amino acids 295-end
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryPAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene Symbol
  • PAK4
  • PAK-4
  • KIAA1142
Protein TargetPAK4
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O96013 # Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments.
SIZE: 591 amino acids; 64072 Da
SUBUNIT: Interacts with FGFR2 and GRB2 (By similarity). Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1.
TISSUE SPECIFICITY: Highest expression in prostate, testis and colon.
PTM: Autophosphorylated on serine residues when activated by CDC42/p21. & Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity).
SIMILARITY: SwissProt: O96013 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.
Molecular Weight61.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP (Catalog #13-104)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-584) and in bulk (cat# 14-584-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-584M 04053252734540

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Alternative Packsize

Número de referencia Descripción
14-584 PAK4 Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases