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2900 Nuclear Extraction Kit

Nuclear Extraction Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Protein Extraction
2900
100 assays  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number2900
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionNuclear Extraction Kit
Materials Required but Not Delivered1. Tissue Culture Reagents

2. Cell Detachment Buffer (Trypsin) or Cell Scrapers

3. Syringes, 1mL - 27 Gauge Needle

4. 1.5 mL Microcentrifuge Tubes

5. 50 mL Conical Tubes

6. Deionized Water

7. Phosphatase Inhibitor (if needed)

8. Microcentrifuge, 4°C

9. Table-top Centrifuge (capable of 16,000 x g), 4°C

10. Rotator/Orbital Shaker, 4°C
References
Product Information
Components
  • Cytoplasmic Lysis Buffer, 10x: - (Part No. 90497) One vial containing 10mL of a concentrated lysis buffer. Dilute to a 1x in deionized water.
  • Nuclear Extraction Buffer: - (Part No. 90498) One vial containing 50mL of a 1x nuclear extraction buffer.
  • PBS Packets: - (Part No. 60093) Two pouches containing enough dry reagent to prepare 1 liter of a 1x PBS solution per pouch. Dilute packet in deionized water.
  • DTT, 1M: - (Part No. 90499) One vial containing 100 μL of 1M Dithiothreitol. Prior to use, dilute to a final concentration of 0.5mM (1:2000) in 1x Cytoplasmic Lysis Buffer and 1x Nuclear Extraction Buffer.
  • Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cell and tissue extract buffers. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators (e.g. EDTA, EGTA). Prior to use, dilute 1/1000 in 1x Cytoplasmic Lysis Buffer and 1x Nuclear Extraction Buffer.
  • Detergent, 10%: - (Part No. 90500) One vial containing 3mL of 10% IGEPAL CA-630 (detergent). Cytoplasmic Lysis Buffer and Nuclear Extraction Buffer already contain detergent, however, under certain conditions, more detergent may be required - refer to Extraction Procedure.
Applications
Key Applications
  • Protein Extraction
Application NotesCHEMICON®'s Nuclear Extraction Kit (Catalog No. 2900) provides a simple and convenient method for the isolation of cytoplasmic and nuclear samples from mammalian cell culture or tissue samples. The Nuclear Extraction Kit can be used in the preparation of purified proteins for use in Western blotting, Electrophoretic Mobility Shift Assays (EMSA), and in CHEMICON®'s Transcription Factor Assay product line.

For Research Use Only; Not for use in diagnostic procedures.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe Nuclear Extraction Kit is shipped and stored at -20ºC.

·Cytoplasmic Lysis Buffer (10x), Nuclear Extraction Buffer (1x), 10% Detergent, and the PBS Packets can be stored at 2-8ºC.

·DTT, 1M and Protease Inhibitor Cocktail must be stored at -20ºC. Avoid repeated freeze-thaw cycles.
Packaging Information
Material Size100 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
2900 08436037124488