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492002 NOX1 Inhibitor, ML171 - CAS 6631-94-3 - Calbiochem

The NOX1 Inhibitor, ML171, also referenced under CAS 6631-94-3, controls the biological activity of NOX1.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: NADPH Oxidase-1 Inhibitor, ML171, 2-APT, 2-acetylphenothiazine

492002
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Descripción

Tabla espec. clave

CAS #Empirical Formula
6631-94-3C₁₄H₁₁NOS

Products

Número de referenciaEmbalaje Cant./Env.
492002-10MG Frasco de vidrio 10 mg
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Description
OverviewA highly selective, cell-permeable, and reversible 2-acetylphenothiazine that is shown to inhibit NADPH Oxidase-1 (Nox1) (IC50 = 0.129 µM, and 0.25 µM) in human HT29 and HEK293 cell-based assays, respectively. Unlike most other currently used Nox inhibitors, it only has marginal activity on other cellular ROS-producing enzymes and receptors including the other Nox isoforms (IC50 = 5 µM, 3 µM and 5 µM for Nox2, Nox3, and Nox4, respectively) and xanthine oxidase, XO, (IC50 = 5.5 µM) in HEK293 cell-based assays. It is also shown to block Nox1-dependent ROS generation in HEK293 cultures dose-dependently at concentrations up to ~ 100 nM; however, its inhibitory effect is reversed through over-expression of Nox1, thereby suggesting that this compound is highly selective for Nox1, with little effect against Nox2-dependent ROS generation (IC50 > 10 µM). Qualitatively, it displays an inhibitory effect against SrcYF-induced ECM-degrading invadopodia formation in DLD1 Human Colon Cancer Cells.

Note: this compound has been reported to interfere with peroxidase-dependent ROS-measuring assays and show no effect on PMA-induced respiratory burst of human granulocytes (Seredenina, T., et al).
Catalogue Number492002
Brand Family Calbiochem®
SynonymsNADPH Oxidase-1 Inhibitor, ML171, 2-APT, 2-acetylphenothiazine
References
ReferencesSeredenina, T., et al. 2015. Free Radic. Biol. Med. 239.
Gianni, D., et al. 2010. ACS Chem. Biol. 5, 981.
Product Information
CAS number6631-94-3
FormDark yellow-orange powder
Hill FormulaC₁₄H₁₁NOS
Chemical formulaC₁₄H₁₁NOS
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Purity≥95% by HPLC
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Número de referencia GTIN
492002-10MG 04055977273465