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XL-85K Multi-Species Leptin RIA

XL-85K
250 tubes  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Leptin H, M, RRIARadioactive 125I
Description
Catalogue NumberXL-85K
DescriptionMulti-Species Leptin RIA
Background InformationLeptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Product Information
Detection methodRadioactive 125I
Performance
  • ED80 = 3.0 ± 0.28 ng/mL HE
  • ED50 = 8.1 ± 0.60 ng/mL HE
  • ED20 = 24.4 ± 2.0 ng/mL HE, HE = Human Equivalent
Precision, %
  • Inter-assay: 6.5–8.7
  • Intra-assay: 2.8–3.6
Quality LevelMQ100
Applications
ApplicationThis radioimmunoassay (RIA) measures & quantifies Leptin for use in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • Radioimmunoassay
Application NotesTwo-day disequilibrium incubationat 4°C. Sample volume 100 µL serum, plasma, or tissue culture media
Biological Information
SpecificityHuman Leptin 100% Porcine Leptin 67% Rat Leptin 61% Mouse Leptin 73% Human Insulin Not Detectable Human Proinsulin Not Detectable Human C-Peptide Not Detectable Rat Insulin Not Detectable Glucagon Not Detectable IGF-I Not Detectable Canine Lepti
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • Leptin
Radioactive Isotype125I-Mouse Leptin
Accuracy
  • 83–104%
Physicochemical Information
Linearity of Dilution94–115%
Standard Curve Range
  • 1–50 ng/mL (Human equivalent)
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size250 tubes
Global Trade Item Number
Número de referencia GTIN
XL-85K 04053252661631