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Microcon® Centrifugal Filters

For DNA and protein concentration

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Descripción

Microcon® Centrifugal Filters

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel® membrane, Microcon® filters offer:

  • Typical recoveries of >95%, even for dilute solutions
  • Reverse spin to maximize recovery, even in the smallest samples
  • Convenient storage of filtrate or concentrated sample in standard microfuge tube
  • Concentration factors up to 100X

Microcon® DNA Fast Flow Filter

Optimized for the concentration and recovery of genomic DNA with SDS buffer. The low nonspecific binding characteristics of the membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

Features:

  • High recovery for small volumes with reverse spin (concentration factor <20X)
  • Low-binding Ultracel® membrane
  • Fast processing

Sample applications:

  • Recovery of genomic DNA for forensic applications
  • Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
  • Removal of primers from amplified DNA
  • Easy filtrate recovery, exploiting the flat membrane design

Microcon® Device
Application 10K           30K           DNA Fast Flow
Peptide and growth factor concentration
Protein concentration and desalting of columns eluates
Protein concentration before electrophoresis or other assays
Protein removal prior to HPLC
Purification of macromolecular components found in tissue culture extracts and cell lysates
Concentration of biological samples (antigens, antibodies, enzymes)
Concentration of gDNA with or without SDS buffer
Concentration and desalting of nucleic acids (single-or double-stranded)
Removal of labeled nucleotides
Removal of labeled amino acids
Removal of primers from amplified DNA
Removal of linkers prior to cloning

Applications

Recovery of Genomic DNA for Forensic Applications, Concentration and Desalting of Nucleic Acids (high recovery alternative to ethanol precipitation), Removal of Primers from Amplified DNA, Protein Concentration