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48-690MAG MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay

This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-690MAG
96-well plate  
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Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
CD3ε (pan Tyr) CREB (S133) ERK (Thr185/Tyr187) LAT (pan Tyr) Lck (pan Tyr) Syk (pan Tyr) ZAP-70 (pan Tyr) HMplexLuminex xMAP
Description
Catalogue Number48-690MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-Assay: 3% - 8%
  • Inter-Assay: 4% - 17%
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationThis cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • CD3ε (pan Tyr)
  • CREB (S133)
  • ERK (Thr185/Tyr187)
  • LAT (pan Tyr)
  • Lck (pan Tyr)
  • Syk (pan Tyr)
  • ZAP-70 (pan Tyr)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
48-690MAG 04053252697210