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HGAMMAG-303E MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
HGAMMAG-303E
96-well plate  96 Well Plate
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IgE HMplexLuminex xMAP
Description
Catalogue NumberHGAMMAG-303E
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
Background InformationFound only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found
primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.

Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
References
Product Information
Components
  • MILLIPLEX® MAP Anti-Human k and λ Light Chain, PE
  • MILLIPLEX® MAP Human IgE Standard
  • MILLIPLEX® MAP Human Serum Positive Control
  • MILLIPLEX® MAP Assay Buffer
  • MILLIPLEX® MAP Washer Buffer, 10X
  • Set of one 96-Well Filter Plate with 4 Sealers
Detection methodLuminex xMAP
ConfigurationSingle Plex
Precision, %
  • Intra-Assay < 10
  • Inter-Assay < 13
Panel TypeMAGNETIC Immune Response
Quality LevelMQ200
Applications
ApplicationIsotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of IgE in human serum, plasma or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well.
Biological Information
SpecificityCross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • IgE
Accuracy
  • IgE 102%
Physicochemical Information
Sensitivity
  • 0.4 ng/mL
Standard Curve Range
  • IgE 4.1 – 1,000 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 Well Plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HGAMMAG-303E 04055977289282

Documentation

MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
The Power of Biomarker Analysis

Licencias necesarias e Información técnica

Cargo
96 well Template 2 Up
96 well Template Single
HGAMMAG-303E xPonent Protocol Template Version Magpix
HGAMMAG-303E xPonent Standard Template Version Magpix
Quality Control Range Sheet- 303E-12K
Quality Control Range Sheet- 303E-13K