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48-621MAG MILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-621MAG
96-well plate  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ATR (Total) Chk1 (Ser345) Chk2 (Thr68) H2A.X (Ser139) MDM2 (total) p21 (Total) p53 (Ser15) H, M, RMplexLuminex xMAP
Description
Catalogue Number48-621MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates:

• ATR (total)
• Chk1 (Ser345)
• Chk2 (Thr68)
• H2A.X (Ser139)
• MDM2 (total)
• p21 (total)
• p53 (Ser15)
References
Product Information
Detection methodLuminex xMAP
HS Code3002 15 90
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • ATR (Total)
  • Chk1 (Ser345)
  • Chk2 (Thr68)
  • H2A.X (Ser139)
  • MDM2 (total)
  • p21 (Total)
  • p53 (Ser15)
Assay PanelEverything you need in a single kit.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
48-621MAG 04053252936067

Documentation

Protocols

Title
Protocol- 48-621MAG

MILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
The Power of Biomarker Analysis

Licencias necesarias e Información técnica

Cargo
96 well Template 2 Up
96 well Template Single
Exploring DNA Damage/Genotoxicity Using the MILLIPLEX®MAP 7-plex DNA Damage/Genotoxicity Kit

Póster

Cargo
Toxicology in drug discovery and development: Biomarkers and model organisms