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109261 Solución de Lugol (solución diluida de yodo y yoduro potásico)

109261
Purchase on Sigma-Aldrich

Descripción

Products

Número de referenciaEmbalaje Cant./Env.
1092611000 Frasco de vidrio 1 l
1092612500 Frasco de vidrio 2.5 l
Descripción
Número de catálogo109261
SinónimosIodine-potassium iodide solution
DescripciónLugol's solution (diluted iodine-potassium iodide solution)
Visión de conjuntoLugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.
Lugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Información del producto
Código HS 3212 90 00
Quality LevelMQ400
Aplicaciones
Aplicación for the Gram staining method
Información fisicoquímica
Punto de ebullición100 °C (1013 hPa)
Densidad1.01 g/cm3 (20 °C)
Valor de pH3.5 (H₂O, 20 °C)
Información de seguridad según el GHS
Clase de almacenamiento10 - 13 Otros liquidos y sustancias sólidas
WGKWGK 2 ponen en peligro significativamente el agua
Información de almacenamiento y transporte
AlmacenamientoAlmacenar entre +15°C y +25°C.
Especificaciones
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red
Global Trade Item Number
Número de referencia GTIN
1092611000 04022536112701
1092612500 04022536557410