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14-630 KDR Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, human KDR amino acids 790-end,
for use in Kinase Assays.

KDR Protein, active, 10 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-630
10 µg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-630
Brand Family Upstate
Trade Name
  • Upstate
DescriptionKDR Protein, active, 10 µg
Overviewhuman KDR expressed by baculovirus in Sf21 insect cells
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, human KDR amino acids 790-end,
for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal His6-tagged, recombinant, human KDR amino acids 790-end
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • KDR
  • VEGFR-2
  • VEGFR2
  • CD309
  • FLK1
  • VEGFR
Protein TargetKDR
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1356 amino acids; 151527 Da
SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight67.9kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of myelin basic protein (MBP)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-630 04053252277849

Documentation

KDR Protein, active, 10 µg Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

KDR Protein, active, 10 µg Certificados de análisis

CargoNúmero de lote
KDR, active - D7SN051U-O D7SN051U-O
KDR, active - D7SN051U-P D7SN051U-P

Preguntas frecuentes

PreguntaRespuesta
Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.
What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.

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Alternative Packsize

Número de referencia Descripción
14-630M KDR Protein, active, 250 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases