Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

IF002 Interferon-γ Protein, Recombinant human

The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.

Interferon-γ Protein, Recombinant human: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Cell Culture UniProt Number: P01579 Entrez Gene Number: NM_000619.2
IF002
100 µg  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Key ApplicationsSpeciesSourcePurity
CULTHuman Human, expressed in E.coliGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
Description
Catalogue NumberIF002
ReplacesIF3
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionInterferon-γ Protein, Recombinant human
OverviewInterferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Product Information
PresentationSterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Quality LevelMQ100
Applications
ApplicationThe Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.
Key Applications
  • Cell Culture
Application NotesFor most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
SourceHuman, expressed in E.coli
Specific ActivityHuman IFN-gamma is fully biologically active when compared to standards. The ED(50) as determined in a viral resistance assay is <= 0.05 ng/mL corresponding to a specific activity of >= 2 x 10(E7) units/mg.
Entrez Gene Number
Gene Symbol
  • IFNG
  • IFN-gamma
  • IFI
  • IFG
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01579 # Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
SIZE: 166 amino acids; 19348 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
PTM: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
DISEASE: SwissProt: P01579 # In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
SIMILARITY: SwissProt: P01579 ## Belongs to the type II (or gamma) interferon family.
Stem Cell Type
  • Mesenchymal Stem Cells
  • Hematopoietic Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Número de referencia GTIN
IF002 04053252284038