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IPVH08130 Membrana Immobilon-P, PVDF; 0,45 µm, hoja de 8,5 cm x 13,5 cm

Transferencia Western, ensayos de adsorción, análisis de aminoácidos y lipopolisacáridos, secuenciación extremo N-terminal proteínas, transferencia pocillos/ranuras, visualización de glucoproteínas

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Aplicaciones clave: Western Blotting, Dot Blot
IPVH08130
10  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Aplicaciones clave
WB, DB
Descripción
Número de catálogoIPVH08130
Nombre comercial
  • Immobilon
DescripciónMembrana Immobilon-P, PVDF; 0,45 µm, hoja de 8,5 cm x 13,5 cm
Información preliminarOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Referencias bibliográficas
Información del producto
Método de detecciónCromogénico Radioactivo Fluorescente Quimioluminiscencía
Código HS 3921 19 00
Tinciones compatibles
  • Azul brillante de Coomassie
  • Negro amido
  • Tinta india
  • Rojo Ponceau-S
  • Oro coloidal
  • CPTS
  • Azul de toluidina
  • Transiluminación
  • Rubí Sypro®
Código del filtroIPVH
Color del filtroBlanco
Quality LevelMQ400
Aplicaciones
Aplicación Transferencia Western, ensayos de adsorción, análisis de aminoácidos y lipopolisacáridos, secuenciación extremo N-terminal proteínas, transferencia pocillos/ranuras, visualización de glucoproteínas
Aplicaciones clave
  • Western Blotting
  • Dot Blot
Información biológica
Mecanismos de adsorción principalesElectrostático, hidrófobo
Adsorción de BSA215 µg/cm²
Adsorción de IgG de cabra294 µg/cm²
Adsorción de insulina160 µg/cm²
HumectabilidadHidrófoba
Información fisicoquímica
Tamaño de poro0.45 µm
Dimensiones
Superficie del filtroLisa
Dimensiones del filtro8.5 cm x 13.5 cm
Información de materiales
Química
  • PVDF hidrófobo
Información toxicológica
Información de seguridad según el GHS
Información de seguridad
Declaraciones de uso del producto
Información de almacenamiento y transporte
Información sobre embalaje
Cantitad10
Información de transporte
Información complementaria
Especificaciones
Global Trade Item Number
Número de referencia GTIN
IPVH08130 04053252283215

Documentation

Membrana Immobilon-P, PVDF; 0,45 µm, hoja de 8,5 cm x 13,5 cm Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
SNAP i.d. 2.0 System Brochure
Western Blotting Tools

Licencias necesarias e Información técnica

Cargo
Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system
Protein Blotting Handbook: 6th Edition

Ficha técnica

Cargo
Immobilon Transfer Membranes: For superior protein and nucleic acid blots

Manuales del usuario

Cargo
Immobilon -P Blotting Sandwiches
Immobilon®-P Transfer Membrane User Guide
User Guide - Immobilon®P Transfer Membrane