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SCR545 Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The Human STEMCCA Cre-Excisable Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.

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SCR545
1 kit  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberSCR545
Trade Name
  • STEMCCA
DescriptionHuman STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
OverviewThe Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of human induced pluripotent stem cells from human foreskin fibroblasts (HFFs). The Cre-excisable STEMCCA vector, comprised of the transcription factors human Oct-4, Klf4, SOX-2, and c-Myc (OKSM) separated by the self-cleaving 2A peptide and IRES sequences, includes flanking LoxP sites that enable Cre-mediated excision of the viral transgenes following reprogramming (Sommer CA, 2009).

The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations. Furthermore, the ability to remove the reprogramming vector has been shown to improve the developmental potential of iPS cells and significantly increase their capacity to undergo directed differentiation in vitro (Sommer CA, 2010).
References
Product Information
Components
  • 1. Human STEMCCA Cre-Excisable Constitutive (OKSM) Lentivirus: (Part number SCR545-1) Two (2) vials of EF1α-hSTEMCCA-LoxP (OKSM) Lentivirus (Part number CS204503). Each vial contains 15 µL of high titer lentivirus.
  • 2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.
Quality LevelMQ100
Applications
ApplicationThe Human STEMCCA Cre-Excisable Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
Biological Information
Stem Cell Type
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsLentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
SCR545 04053252013812

Documentation

Referencias bibliográficas

Visión general referenciasPub Med ID
Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
Kadari, Asifiqbal, et al.
Stem Cell Res Ther, 5: 47 (2014)  2014

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Folleto

Cargo
Human Stem Cell Systems
Simplifying the iPSC Workflow
Stem Cell Research

Licencias necesarias e Información técnica

Cargo
Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Ficha técnica

Cargo
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
STEMCCA Lentivirus Reprogramming Kits

Póster

Cargo
Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein

Manuales del usuario

Cargo
Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit