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03-228 HDAC2 full length (His-tag) human recombinant

Human recombinant HDAC2 full length (His-tag) produced in insect cells.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Enzyme Assays
03-228
50 µg  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Key ApplicationsSpecies
Enzyme AssaysHuman
Description
Catalogue Number03-228
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHDAC2 full length (His-tag) human recombinant
OverviewHuman recombinant HDAC2 full length (His-tag) produced in insect cells
Background InformationHistone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely involved in regulating chromatin structure during transcription. It associates with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA. This process is highly conserved from yeast to human.
Product Information
FormatPurified
PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant HDAC2 full length (His-tag) produced in insect cells.
Key Applications
  • Enzyme Assays
Application NotesHDAC deacetylase assays
Biological Information
PurityPurity >50% by SDS-PAGE
SourceFull length human HDAC2
Specific Activity> 1U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
Molecular Weight~66 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.
Packaging Information
Material Size50 µg
Global Trade Item Number
Número de referencia GTIN
03-228 04053252550898