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13-118 GST-NFκB p50 subunit (δ434-969)

N-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography.

GST-NFκB p50 subunit (δ434-969): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
UniProt Number: P19838 Entrez Gene Number: NM_003998.2
13-118
50 µg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number13-118
Brand Family Upstate
Trade Name
  • Upstate
DescriptionGST-NFκB p50 subunit (δ434-969)
OverviewN-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography. MW = 74kDa.
References
Product Information
Presentation50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% βmercaptoethanol, 0.03% Brij-35, 0.2mM PMSF, 1mM benzamidine and 50% glycerol
Quality LevelMQ100
Applications
ApplicationN-terminal GST-tagged, recombinant human NFkB, p50 subunit (D434-969), expressed in E. coli. Purified using glutathione agarose chromatography.
Application NotesDNA Binding Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
Gene Symbol
  • NFKB1
  • NF-kappa-B
  • DKFZp686C01211
  • MGC54151
  • NFKB-p105
  • NFKB-p50
  • EBP-1
  • KBF1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P19838 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. Plays a role in the regulation of apoptosis.
SIZE: 968 amino acids; 105356 Da
SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
DOMAIN: SwissProt: P19838 The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation. & Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Polyubiquitination seems to allow p105 processing.
SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated in a DNA binding assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
13-118 04053252371875

Documentation

GST-NFκB p50 subunit (δ434-969) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

GST-NFκB p50 subunit (δ434-969) Certificados de análisis

CargoNúmero de lote
GST-NFkB p50 subunit (D434-969) - DAM1520788 DAM1520788
GST-NFkappa;B p50 subunit (Delta;434-969) - 26844 26844
GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) - 2384037 2384037
GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) 3054689
GST-NFκB p50 subunit (Δ434-969) (recombinant protein expressed in E. coli) 2879688
GST-NFκB p50 subunit (Δ434-969) - 3541438 3541438
GST-NFκB p50 subunit (Δ434-969) - 3991569 3991569
GST-NFκB p50 subunit (Δ434-969) - 4163778 4163778