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14-752 GRK7 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

GRK7 Protein, active, 10 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-752
10 µg  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-752
Brand Family Upstate
Trade Name
  • Upstate
DescriptionGRK7 Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
References
Product Information
Presentation10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceRecombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
Specific Activity381U/mg, where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation.
Gene Symbol
  • GRK7
  • GPRK7
Protein TargetGRK7
Purification MethodPurified using Ni2+/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q8WTQ7 # Phosphorylates cone opsins thereby initiating their deactivation.
SIZE: 553 amino acids; 62212 Da
SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
TISSUE SPECIFICITY: Retina. Cones and rod.
SIMILARITY: SwissProt: Q8WTQ7 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain.
Molecular Weight66 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-752 04053252516214

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Alternative Packsize

Número de referencia Descripción
14-752M GRK7 Protein, active, 250 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases