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344935 Furin Substrate, Fluorogenic - Calbiochem

A fluorogenic substrate for furin, a mammalian homolog of the yeast Kex2 endoprotease (kcat/Km ~ 2 x 10⁴ s⁻¹M⁻¹).

Furin Substrate, Fluorogenic - Calbiochem: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
344935
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Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
344935-5MG Ampolla de plást. 5 mg
Description
OverviewA fluorogenic substrate for furin, a mammalian homolog of the yeast Kex2 endoprotease (kcat/Km ~ 2 x 104 s-1M-1).
Catalogue Number344935
Brand Family Calbiochem®
References
ReferencesLamango, N.S., et al. 1996. Arch. Biochem. Biophys. 330, 238.
Hatsuzawa, K., et al. 1992. J. Biol. Chem. 267, 16094.
Product Information
ATP CompetitiveN
FormWhite solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₃₇H₅₇N₁₃O₉
Chemical formulaC₃₇H₅₇N₁₃O₉
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorogenic substrate for furin
Primary Target IC<sub>50</sub>kcat/Km ~ 2 x 104 s-1M-1 for furin
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequencePyr-Arg-Thr-Lys-Arg-AMC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
344935-5MG 04055977194418

Documentation

Furin Substrate, Fluorogenic - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Furin Substrate, Fluorogenic - Calbiochem Certificados de análisis

CargoNúmero de lote
344935

Referencias bibliográficas

Visión general referencias
Lamango, N.S., et al. 1996. Arch. Biochem. Biophys. 330, 238.
Hatsuzawa, K., et al. 1992. J. Biol. Chem. 267, 16094.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-June-2008 RFH
DescriptionA fluorogenic substrate for furin (kcat/Km ~2 X 104 M-1 s-1), a mammalian homolog of the yeast Kex2 endoprotease. Also serves as a substrate for related protein, prohormone convertase 2 (PC2) (kcat/Km ~6 X 103 M-1 s-1). Excitation max: ~360-380 nm; emission max: ~430-460 nm).
FormWhite solid
FormulationSupplied as a trifluoroacetate salt.
Recommended reaction conditions20 nM of substrate in a 250 µl final reaction volume. Buffer:100 mM MES/NaOH, 1 mM CaCl2, pH 7.0. Temperature: 37°C. Duration of incubation: 1-4 h. Reaction can be terminated by adding 3 ml of 5 mM EDTA and the amount of AMC released is measured.
Chemical formulaC₃₇H₅₇N₁₃O₉
Peptide SequencePyr-Arg-Thr-Lys-Arg-AMC
Purity≥95% by HPLC
SolubilityH₂O (10 mg/ml) or DMSO
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLamango, N.S., et al. 1996. Arch. Biochem. Biophys. 330, 238.
Hatsuzawa, K., et al. 1992. J. Biol. Chem. 267, 16094.