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HC08 Fluorescent Mounting Media, Aqueous

HC08
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
HC08-8ML Frasco de vidrio 8 ml
Description
OverviewAn aqueous mounting medium designed to preserve and enhance the labeling of immunoreactivity in tissue sections. Sustains the fluorescent signal from labeled samples on slides. Inclusion of 4,6-diamidino-2-phenylindole (DAPI; Cat. No. 268298) allows for the visualization of unlabeled (non-apoptotic) cells. Suitable for use with a wide variety of fluorophores.
Catalogue NumberHC08
Brand Family Calbiochem®
References
Product Information
FormLiquid
Formulation8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).
PreservativeNone
Applications
Application NotesImmunofluorescence (see comments)
Application CommentsFor best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HC08-8ML 04055977228359

Documentation

Fluorescent Mounting Media, Aqueous Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Fluorescent Mounting Media, Aqueous Certificados de análisis

CargoNúmero de lote
HC08
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
ApplicationImmunofluorescence (see comments)
DescriptionAqueous mounting media designed to preserve and enhance the labeling of tissue sections or cell preparations fixed on slides. Suitable for many fluorophores including fluorescein, Oregon Green™, Alexa™ 488, rhodamine, Texas Red, Cyanine (Cy™) 3, and Alexa™ 546. Inclusion of DAPI in the Fluorescent Mounting Media allows for the visualization of all cells at an excitation wavelength of 330-380 nm.
FormLiquid
Formulation8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).
PreservativeNone
CommentsFor best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling