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GF144 Epidermal Growth Factor Protein, Human recombinant

The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
UniProt Number: P01133 Entrez Gene Number: NM_001963.2
GF144
500 µg  
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

SourcePurity
E.coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
Description
Catalogue NumberGF144
ReplacesGF001
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionEpidermal Growth Factor Protein, Human recombinant
OverviewEpidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Alternate Names
  • EGF
Product Information
PresentationLyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
Quality LevelMQ100
Applications
ApplicationThe Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
Application NotesFor most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
Biological Information
PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
SourceE.coli
Specific ActivityHuman EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
Entrez Gene Number
Entrez Gene SummaryEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Gene Symbol
  • EGF
  • URG
  • urogastrone
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
SIZE: 1207 amino acids; 133946 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
Stem Cell Type
  • Neural Stem Cells
  • Epithelial Cells
  • Mesenchymal Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
Packaging Information
Material Size500 µg
Global Trade Item Number
Número de referencia GTIN
GF144 08436037123825