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14-674M EphB1 Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, human EphB1 amino acids 564-end, for use in Kinase Assays.

14-674M
250 µg  Also available in 10 μg size (cat#14-674) and in bulk (cat# 14-674-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-674M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionEphB1 Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, human EphB1 amino acids 564-end
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human EphB1 amino acids 564-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryEphrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Gene Symbol
  • EPHB1
  • Hek6
  • EPHT2
  • NET
  • Elk
  • FLJ37986
  • ELK
  • EphB1
  • HEK6
Protein TargetEphB1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P54762 # Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.
SIZE: 984 amino acids; 109885 Da
SUBUNIT: The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Preferentially expressed in brain.
SIMILARITY: SwissProt: P54762 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. & Contains 1 SAM (sterile alpha motif) domain.
Molecular Weight51.4kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of 250μM Src Substrate Peptide (Catalog # 12-140
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-674) and in bulk (cat# 14-674-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-674M 04053252478796

Documentation

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Alternative Packsize

Número de referencia Descripción
14-674 EphB1 Protein, active, 10 µg

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Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases