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S-004-C Enzyme Free Cell Dissociation Solution Hank's Based (1X), liquid, 100ml

The Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 100ml format.

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S-004-C
100 mL  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberS-004-C
Trade Name
  • Specialty Media
DescriptionEnzyme Free Cell Dissociation Solution Hank's Based (1X), liquid, 100ml
OverviewEnzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents and agents to stabilize their activity on cells. This reagent does NOT contain porcine trypsin or any other protein. It works significantly faster than 0.5mM EDTA. In fact, it works as quickly as trypsin on most cells. Most importantly, it preserves the structural and functional integrity of cell surface proteins and does not have the cytotoxic effects sometimes associated with 0.5 mM EDTA. It can be used to dissociate primary cells, tissues, and tumors with an increased plating efficiency.



Enzyme-Free dissociation solution should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, we recommend trypsinizing the cells and replating at 30-40% confluency 24 hours before enzyme-free use (this will allow the cells to be 60-80% confluent for enzyme free dissociation).
References
Product Information
PresentationProduct is supplied as a sterile liquid
Quality LevelMQ200
Applications
ApplicationThe Enzyme-Free Cell Dissociation Solution is a Hank's based formulation of chelating agents & agents to stabilize their activity on cells. This solution is available in a 100ml format.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsProduct can be stored at room temperature or at 4°C. Shelf life is 2 years.
Packaging Information
Material Size100 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
S-004-C 04053252359736