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ECM542 ECM Cell Culture Optimization Array (Colorimetric, 48-Wells)

The ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell gro

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ECM542
1 kit  1 plate, 48-wells
Purchase on Sigma-Aldrich

Descripción

Tabla espec. clave

Detection Methods
Colorimetric
Description
Catalogue NumberECM542
DescriptionECM Cell Culture Optimization Array (Colorimetric, 48-Wells)
OverviewExtracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) can be a time-consuming and labor-intensive process. The 48-well, colorimetric ECM Cell Culture Optimization Array provides a multiparametric assay that allows researchers to: (1) quickly identify ECM(s) that best promote cell-type specific adhesion and (2) pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type.

How does the ECM Cell Culture Optimization Array work?
The foundation of our kit is a 48-well microtiter plate containing several commonly-used ECM proteins. Collagen I, laminin, fibronectin, and vitronectin are arrayed in triplicate with concentrations ranging from 0.1 μg/mL to 20 μg/mL. You simply culture your cells on the plate, and use the included reagents to block, stain, and analyze the adhesion capacity with a plate reader. We have extensively tested and optimized this kit on a variety of cell types including HEK293 cells, and human and rodent neural stem cells.
Materials Required but Not Delivered• Multi-channel and/or repeating pipettes
•15 mL or 50 mL conical tube.
• Harvesting buffer: 2.5 mM EDTA/PBS or Accutase™ (Catalog No. SCR005)
• Tissue culture growth medium appropriate for subject cells, such as DMEM containin10% FBS
• Serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media)
• Sterile 1X PBS or 1X HBSS to wash cells
• Deionized water
• Low speed centrifuge and falcon tubes for cell harvesting
• Cell culture incubator
• Sterile cell culture hood
• Hemacytometer and microscope for counting cells
• Trypan Blue or equivalent viability stain
• Microplate reader (540-570 nm detection) or spectrophotometer
Product Information
Components
  • • ECM Gradient Array Plate: One 48-well plate
  • • 30%BSA: (Part No. CS201638) One vial - 0.5mL
  • • Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
  • • Extraction Buffer: (Part No. 90145) One bottle - 20 mL
  • • Assay Buffer: (Part No. 2003646) One bottle - 100 mL
Detection methodColorimetric
Quality LevelMQ100
Applications
ApplicationThe ECM cell culture Optimization Array enables researchers to identify the best ECM protein for their cell culture environment & also pinpoint the concentration needed to achieve optimal cell growth conditions.
Application NotesThe ECM Cell Culture Optimization Array is the first commercially-available tool of its kind to enable researchers to not only quickly identify the best ECM protein for their cell culture environment, but also pinpoint the concentration needed to achieve optimal cell growth conditions.
Biological Information
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mouse Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe ECM Gradient Array Plate should be stored in the foil pouch at -70ºC, and used within three months of the date of receipt. The remaining kit components should be stored at 2º to 8ºC.
Packaging Information
Material Size1 kit
Material Package1 plate, 48-wells
Global Trade Item Number
Número de referencia GTIN
ECM542 04053252006371