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SCR128 Dopaminergic Differentiation Growth Factor Sampler

SCR128
1 Kit  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

SourcePurity
E. coli.Recombinant growth factors are endotoxin free (<0.1 ng/μg of protein (<1 EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses.
Description
Catalogue NumberSCR128
DescriptionDopaminergic Differentiation Growth Factor Sampler
Background InformationEMD Millipore’s Dopaminergic Differentiation Growth Factor Sampler contains a collection of five validated growth factors that are routinely used to induce differentiation of human pluripotent ES/iPS cells to dopaminergic neurons along with a reliable high affinity antibody marker, anti-tyrosine hydrodylase, to aid in quantifying the percentage yields from differentiation experiments.
References
Product Information
Components
  • 1. Sonic HedgeHog (Shh), Human Recombinant (Part No. GF174-5UG): Two 5 μg vials. Lyophilized powder. Store at -20°C.
  • 2. Fibroblast Growth Factor-8 (FGF-8), Human Recombinant (Part No. GF110-5UG): One 5 μg vial. Lyophilized powder. Store at -20°C.
  • 3. Brain Derived Neurotrophic Factor (BDNF), Human Recombinant (Part No. GF029-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
  • 4. Glial Derived Neurotrophic Factor (GDNF), Human Recombinant (Part No. GF030-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
  • 5. Transforming Growth Factor β-III (TGF-β-III), Human Recombinant (Part No. GF176-2UG): One 2 μg vial. Lyophilized powder. Store at -20°C.
  • 6. Anti-Tyrosine Hydroxylase antibody, rabbit polyclonal (Part No. AB152-20): One 20 μL vial. Store at -20°C.
Quality LevelMQ100
Applications
ApplicationThis Dopaminergic Differentiation Growth Factor Sampler contains five validated growth factors used to induce differentiation of human pluripotent Embryonic stem (ES) & induced pluripotent Stem (iPS) cells to dopaminergic neurons.
Biological Information
PurityRecombinant growth factors are endotoxin free (<0.1 ng/μg of protein (<1 EU/μg) and greater than 98% pure by SDS-PAGE gel and HPLC analyses.
SourceE. coli.
Cross ReactivityNone
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceUsing the growth factors supplied in this kit a 3-5 fold increase of dopaminergic differention (up to 50-70% TH+ cells) was routinely produced using HES/hips cell derived neural progenitor cells vs. spontaneous neuronal differentiation.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
SCR128 04053252551215

Documentation

Dopaminergic Differentiation Growth Factor Sampler Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Folleto

Cargo
Neuroscience Product Selection Guide
Simplifying the iPSC Workflow

Licencias necesarias e Información técnica

Cargo
Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Manuales del usuario

Cargo
Human Dopaminergic Neurogenesis Kit & Dopaminergic Differentiation Growth Factor Sampler