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14-649M DMPK Protein, active, 250 µg

Active, 6His-tagged, recombinant, human DMPK, amino acids 1-549, for use in Kinase Assays.

DMPK Protein, active, 250 µg: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
14-649M
250 µg  Also available in 10 μg size (cat#14-649) and in bulk (cat# 14-649-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-649M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionDMPK Protein, active, 250 µg
Overview6His-tagged, recombinant, human DMPK, amino acids 1-549
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, 6His-tagged, recombinant, human DMPK, amino acids 1-549, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Gene Symbol
  • DMPK
  • DM-kinase
  • MDPK
  • MT-PK
  • DM
  • DM1
  • DMK
  • DM1PK
Protein TargetDMPK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q09013 # Critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity. Phosphorylates phospholamban.
COFACTOR: Magnesium.
SIZE: 639 amino acids; 70357 Da
TISSUE SPECIFICITY: Most isoforms are expressed in many tissues including heart, skeletal muscle, liver and brain, except for isoform 2 which is only found in the heart and skeletal muscle, and isoform 14 which is only found in the brain, with high levels in the striatum, cerebellar cortex and pons.
DISEASE: SwissProt: Q09013 # Defects in DMPK are the cause of myotonic dystrophy 1 (DM1) [MIM:160900]; also known as Steinert disease. DM is an autosomal dominant neurodegenerative disorder characterized by myotonia, muscle wasting in the distal extremities, cataract, hypogonadism, defective endocrine functions, male baldness, and cardiac arrhythmias. DM patients show decreased levels of kinase expression inversely related to repeat length. The minimum estimated incidence is 1 in 8'000 live births. DM1 is caused by a CTG expansion in the 3'-UTR of the DMPK gene. The repeat length usually increases in successive generations, but not always.
SIMILARITY: SwissProt: Q09013 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
Molecular Weight65kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of Axltide (12-516)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-649) and in bulk (cat# 14-649-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-649M 04053252411779

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Alternative Packsize

Número de referencia Descripción
14-649 DMPK Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases