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HTS090M ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation

Human OT GPCR membrane prepation for Radioligand binding Assays.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Host Cell: Chem-1 Uni Prot Number: P30559 Entrez Gene Number: NM_000916.3
HTS090M
200 units  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

SpeciesHost CellsProtein Target
Human Chem-1OT
Description
Catalogue NumberHTS090M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation
OverviewHuman Oxytocin Receptor
Background InformationOxytocin is a cyclic 9 amino acid peptide that differs from vasopressin in 2 amino acids. Despite the close similarities in sequence, oxytocin and vasopressin have different biological activities and bind to distinct G protein-coupled receptors. The oxytocin receptor, OT, couples primarily to Gq/11 to mobilize intracellular calcium. In female reproduction, oxytocin promotes uterine contraction and lactation; oxytocin is the most commonly used drug for induction of labor, whereas an oxytocin antagonist, atosiban, is under investigation to suppress preterm labor. Oxytocin/OT interaction in the CNS also plays an important role in stress, male and female sexual response, and sociality (Gimpl and Fahrenholz, 2001). Chemicon's oxytocin membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of oxytocin receptor interactions with oxytocin. The membrane preparations exhibit a Kd of 0.53nM for [125I]-OVTA. With 5.0 ug/well Oxytocin Membrane Prep and 0.5 nM [125I]-OVTA, a greater than 6-fold signal-to-background ratio was obtained.
References
Product Information
FormatMembranes
PresentationOne package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 6-fold signal:background with 125I-labeled OVTA at 0.5 nM.

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Quality LevelMQ100
Applications
ApplicationHuman OT GPCR membrane prepation for Radioligand binding Assays.
Key Applications
  • Radioligand binding assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to the G-protein coupled receptor family and acts as a receptor for oxytocin. Its activity is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. The oxytocin-oxytocin receptor system plays an important role in the uterus during parturition.
Gene Symbol
  • OXTR
  • OT-R
GPCR ClassA
Host CellsChem-1
Protein TargetOT
Target Sub-FamilyOxytocin
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P30559 # Receptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol- calcium second messenger system.
SIZE: 389 amino acids; 42772 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P30559 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsMembranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] OVTA (Perkin Elmer # NEX254)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Oxytocin Receptor membrane prep:
Product Usage Statements
5 µg/well 10 µg/well
Signal:Background 9 9
Specific Binding (cpm) 21871 24926


SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 4.5 pmol/mg
Kd: 0.53 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HTS090M 04053252475900

Documentation

ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

ChemiScreen™ Recombinant Human OT Oxytocin Receptor Membrane Preparation Certificados de análisis

CargoNúmero de lote
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN OT OXYTOCIN RECEPTOR - 2135594 2135594
CHEMISCREENTM MEMBRANE PREPARATION RECOMBINANT HUMAN OT OXYTOCIN RECEPTOR - 2210074 2210074

Referencias bibliográficas

Visión general referenciasPub Med ID
The oxytocin receptor system: structure, function, and regulation
Gimpl, G and Fahrenholz, F
Physiol Rev, 81:629-683 (2001)  2001

11274341 11274341

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Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Membrane Preparations
Life Science Research > Proteins and Enzymes > GPCR Membrane Preparations