Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

219461 Cathepsin K, His•Tag®, Human, Recombinant, E. coli

Cathepsin K, His•Tag®, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
219461
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Products

Número de referenciaEmbalaje Cant./Env.
219461-25UG Ampolla de plást. 25 μg
Description
OverviewCathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag® sequence.
Note: 1 mU = 1 milliunit.
Catalogue Number219461
Brand Family Calbiochem®
References
ReferencesDelaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
Hou, W.S., et al. 2003. Biol. Chem. 384, 891.
Product Information
CAS number94716-09-3
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
EC number3.4.22.38
FormLiquid
FormulationIn 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥900 mU/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
219461-25UG 04055977218442

Documentation

Cathepsin K, His•Tag®, Human, Recombinant, E. coli Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Cathepsin K, His•Tag®, Human, Recombinant, E. coli Certificados de análisis

CargoNúmero de lote
219461

Referencias bibliográficas

Visión general referencias
Delaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
Hou, W.S., et al. 2003. Biol. Chem. 384, 891.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-March-2013 JSW
DescriptionCathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts. Recently reported to also be expressed in fibroblasts. Irregularities in activity contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. Contains a C-terminal His•Tag®.
FormLiquid
FormulationIn 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number94716-09-3
EC number3.4.22.38
Purity≥95% by SDS-PAGE
Specific activity≥900 mU/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesDelaisse, J.M., et al. 2003. Microsc. Res. Tech. 61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct. 21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int. 73, 380.
Hou, W.S., et al. 2003. Biol. Chem. 384, 891.