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14-663M CaM Kinase I Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant, human CaMK1, amino acids 2-end. For use in Kinase Assays.

14-663M
250 µg  Also available in 10 μg size (cat#14-663) and in bulk (cat#14-663-K).
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number14-663M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCaM Kinase I Protein, active, 250 µg
OverviewN-terminal GST-tagged, recombinant, human CaMK1, amino acids 2-end.
References
Product Information
Presentation50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged, recombinant, human CaMK1, amino acids 2-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli cells
Entrez Gene Number
Entrez Gene SummaryCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.
Gene Symbol
  • CAMK1
  • CaMKI-alpha
  • MGC120318
  • CAMK1-PEN
  • CAMKI
  • CaM-KI
  • CaMKI
  • MGC120317
Protein TargetCaMKI
Purification MethodGlutathione-agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14012 # Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium- dependent activation of the ERK pathway (By similarity). Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]- x(3)-[MVLIF]. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CTFR, MYL9, SYN1/synapsin I and SYNII/synapsin II (By similarity).
SIZE: 370 amino acids; 41337 Da
SUBUNIT: Monomer. Interacts with XPO1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly cytoplasmic (By similarity).
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN:SwissProt: Q14012 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
Molecular Weight68 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of ZIPtide (#12-545).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-663) and in bulk (cat#14-663-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
14-663M 04053252924637

Documentation

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Número de referencia Descripción
14-663 CaM Kinase I Protein, active, 10 µg

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Categorías

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases