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SCT110 BioTracker 488 Green CSFE Cell Proliferation Kit

Live cell imaging cell proliferation kit based on carboxyfluorescein succinimidyl ester (CFSE) used for fluorescent cell labeling in flow cytometry applications.

SCT110
1 Kit  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue NumberSCT110
DescriptionBioTracker 488 Green CSFE Cell Proliferation Kit
OverviewBioTracker SE Cell Proliferation Kits diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters of amine-reactive dyes, but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling is revealed by the appearance of a successively dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining can withstand fixation and permeabilization for subsequent immunostaining. Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader.

Spectral Properties
Absorbance: 495nm
Emission: 519nm
Alternate Names
  • Live cell imaging probe
References
Product Information
Components
  • 1. 10 vials of BioTracker 488 Green CFSE Dye (50µg) (CS224588)
  • 2. 1 vials of Anhydrous DMSO (500µL) (CS224578)
Detection methodFluorescence
PresentationLiquid
Applications
ApplicationLive cell imaging cell proliferation kit based on carboxyfluorescein succinimidyl ester (CFSE) used for fluorescent cell labeling in flow cytometry applications.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSpectral Properties
Absorbance: 495nm
Emission: 519nm
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker 488 Green CSFE Cell Proliferation Kit -20ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening. Ideally the 5 mM DMSO stock solution should be prepared on the day of use. Aliquots may be stored for later use, but activity may be reduced over time. The dyes should only be added to aqueous buffer immediately before staining.
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
SCT110 04054839566080