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71546 BacMagic™ Transfection Kit - Novagen

BacMagic™ Transfection Kit - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
71546
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Número de referenciaEmbalaje Cant./Env.
71546-3 Ampolla de plást. 5 rxn
Description
OverviewThe BacMagic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. BacMagic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTriEx™ vectors, is cotransfected with BacMagic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The BacMagic™-1 Transfection Kit contains the BacMagic™-1 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector® Medium, for optimal transfection performance.
Catalogue Number71546
Brand Family Novagen®
Application Data
Figure 1. Construction of baculovirus recombinants with three BacMagic systems.

Figure 2. Streamlined baculovirus method
References
Product Information
•0.5 µgBacMagic DNA
•50 µlInsect GeneJuice Transfection Reagent
•2 x 1 mlSf9 Cells
•1 LBacVector® Insect Cell Medium
•2 µgTransfection Control Plasmid
Quality LevelMQ100
Applications
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Toxicological Information
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Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
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Specifications
Global Trade Item Number
Número de referencia GTIN
71546-3 04055977252415