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SCC165 BLaER1 Human B-cell Precursor Leukemia Cell Line

BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Key Applications: Cell Culture
SCC165
≥1X10⁶ cells/vial   
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Descripción

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Key Applications
CULT
Description
Catalogue NumberSCC165
DescriptionBLaER1 Human B-cell Precursor Leukemia Cell Line
Alternate Names
  • B cell Leukemia C/EBPalphaER clone 1
Background InformationInduction of differentiation is a therapeutic approach for cancer. Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective (1). Ectopic expression of the transcription factor C/EBPα causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype.

The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPα fused with the estrogen receptor hormone binding domain (ER) and GFP (2,3). A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages (2). Cells retained their phenotype even when C/EBPα was inactivated, a characteristic of genuine cell reprogramming. C/EBPα induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.

Source:
BLaER1 cells are a single subclone isolated from C/EBPαER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 (4).

Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.

References:
1. Waxman, S. (2000). Leukemia 14(3):491-496.
2. Rapino F et al. (2013). Cell Reports 3(4): 1153-1163.
3. Rapino F et al. (2017). Cell Reports 19(6): 1281.
4. Jack I et al. (1986). Cancer Genet. Cytogenet. 19(3-4):261-269.
References
Product Information
Applications
ApplicationBLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.
• Cells tested positive for squirrel monkey retrovirus (SMRV).
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
SCC165 04054839573750

Documentation

BLaER1 Human B-cell Precursor Leukemia Cell Line Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Referencias bibliográficas

Visión general referenciasPub Med ID
CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response
Grégoire Stik, <br />Enrique Vidal, <br />Mercedes Barrero, <br />Sergi Cuartero, <br />Maria Vila-Casadesús, <br />Julen Mendieta-Esteban, <br />Tian V. Tian, <br />Jinmi Choi, <br />Clara Berenguer, <br />Amaya Abad, <br />Beatrice Borsari, <br />François le Dily, <br />Patrick Cramer, <br />Marc A. Marti-Renom, <br />Ralph Stadhouders & <br />Thomas Graf
Nature Genetics  2  655–661  2020

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Ficha técnica

Cargo
BLaER1 Human B-cell Precursor Leukemia Cell Line