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ABS1600 Anti-phospho-UVRAG (Ser498)

Detect UV radiation resistance-associated gene protein using this rabbit polyclonal Anti-phospho-UVRAG (Ser498), Cat. No. ABS1600 that has been tested in Western Blotting.

Anti-phospho-UVRAG (Ser498): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Gene Symbol: UVRAG UniProt Number: Q9P2Y5
ABS1600
100 µL  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1600
DescriptionAnti-phospho-UVRAG (Ser498)
Alternate Names
  • UV radiation resistance-associated gene protein
  • p63
Background InformationUV radiation resistance-associated gene protein (UniProt: Q9P2Y5; also known as p63) is encoded by the UVRAG gene (Gene ID: 7405) in human. UVRAG is a key autophagic tumor suppressor that is mutated in several common human cancers. It contains four functional domains, a proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain. It is a critical regulator of intracellular membrane trafficking, autophagy, and chromosomal stability. UVRAG is also involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex. Its function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns3P). During autophagy UVRAG acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. It is involved in maturation of autophagosomes and degradative endocytic trafficking independently of Beclin-1, but depends on its association with a class C Vps complex. UVRAG is also shown to enhance class C Vps complex association with a SNARE complex and promote fusogenic SNARE complex formation during late endocytic membrane fusion. UVRAG is also reported to promote DNA double-strand break repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining. Disruption of UVRAG is shown to increase genetic instability and sensitivity of cells to irradiation. (Ref: Zhao, Z et al. (2012). Dev. Cell 22(5): 1001-1016).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect UV radiation resistance-associated gene protein using this rabbit polyclonal Anti-phospho-UVRAG (Ser498), Cat. No. ABS1600 that has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected phospho-UVRAG (Ser498) in TSC1 KO MEFs and treated with Torin1 (Kim, Y.M., et. al. (2015). Mol Cell. 57(2):207-18).

Western Blotting Analysis: A representative lot detected phospho-UVRAG (Ser498) in HA-UVRAG+myc-Rheb2+DMSO. (Courtesy of Dr. Do-Hyung Kim at University of Minnesota).
Biological Information
ImmunogenA linear peptide corresponding to 15 amino acids from the C-terminal half of human UVRAG surrounding phosphoserine 498.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects human UVRAG phosphorylated on Serine 498.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UVRAG
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~98 kDa observed; 78.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with HA-UVRAG+myc-Rheb2+DMSO (vehicle).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-UVRAG (Ser498) in HEK293 cells transfected with HA-UVRAG+myc-Rheb2+DMSO (vehicle).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ABS1600 04054839193569