Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

05-773 Anti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal

05-773
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, HWBRbPurifiedMonoclonal Antibody
Description
Catalogue Number05-773
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-MYPT1 (Thr850) Antibody, clone SA19, rabbit monoclonal
Product Information
FormatPurified
Quality LevelMQ100
Applications
ApplicationAnti-phospho-MYPT1 (Thr850) Antibody, clone SA19 is an antibody against phospho-MYPT1 (Thr850) for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...RS[pT]GV..., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
CloneSA19
HostRabbit
SpecificityMYPT1 phosphorylated on Thr850
IsotypeIgG
Species Reactivity
  • Chicken
  • Human
Species Reactivity NotePredicted to cross react with mouse and rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
Gene Symbol
  • PPP1R12A
  • MYPT1
  • MGC133042
  • MBS
Modifications
  • Phosphorylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.
Molecular Weightdoublet at 130kDa
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment
Packaging Information
Material Size100 µL
Global Trade Item Number
Número de referencia GTIN
05-773 04053252624681