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07-1414 Anti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody

This Anti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody is validated for use in WB, ELISA for the detection of phospho-Glycogen Synthase (muscle) (Ser640).

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Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: GYS1, Glycogen, GYS, GSY UniProt Number: P13807
07-1414
100 µg  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ELISARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1414
DescriptionAnti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody
Alternate Names
  • Starchsynthase, muscle
Background InformationA glycosyltransferase enzyme that catalyses the reaction of UDP-glucose and (1,4-α-D-glucosyl)n to yield UDP and (1,4-α-D-glucosyl)n+1. In other words, this enzyme converts excess glucose residues one by one into a polymeric chain for storage as glycogen. The reaction is highly regulated by allosteric effectors such as glucose-6-phosphate, by phosphorylation reactions, and indirectly triggered by the hormone insulin.
References
Product Information
FormatAffinity Purified
Control
  • PDGF treated NIH/3T3 cell lysate
PresentationPurified rabbit polyclonal IgG in buffer containing 0.02 M Potassium phosphate, pH 7.2 with 0.15 M Sodium chloride and 0.01% sodium azide.
Applications
ApplicationThis Anti-phospho-Glycogen Synthase (muscle) (Ser640) Antibody is validated for use in WB, ELISA for the detection of phospho-Glycogen Synthase (muscle) (Ser640).
Key Applications
  • Western Blotting
  • ELISA
Application NotesDirect ELISA: 1:30,000-1:150,000 dilution of a previous lot was used in direct ELISA when assayed against 0.1 µg of the immunogen peptide.

Optimal working dilutions must be determined by the end user.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Biological Information
ImmunogenSynthetic phospho peptide from human muscle Glycogen synthase.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityGlycogen synthase (muscle), phosphoSer640. By Western blot the antibody recognizes the ~84 kDa phosphorylated protein. Reactivity to non-phosphorylated human Glycogen synthase is minimal (less than 0.2%) by ELISA.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGlycogen is a high molecular mass polysaccharide that serves as a repository of glucose for use in times of metabolic need. Glycogen synthase (EC 2.4.1.11) catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages (Pederson et al., 2004 [PubMed 15282316]).[supplied by OMIM]
Gene Symbol
  • GYS1
  • Glycogen
  • GYS
  • GSY
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
CATALYTIC ACTIVITY: UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).
ENZYME REGULATION: Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does (By similarity).
PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
DISEASE: Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also called muscle glycogen synthase deficiency. GSD0 is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work.
SIMILARITY: Belongs to the mammalian/fungal glycogen synthase family.
Molecular WeightAntibody recognizes the ~84 kDa phosphorylated protein.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on PDGF treated NIH/3T3 lysates.

Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected phosphorylated Glycogen Synthase on 10 μg of PDGF treated NIH/3T3 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -20°C in undiluted aliquotes from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
07-1414 04053252589126