Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

MABE636 Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140

This Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B UniProt Number: P84243
MABE636
100 μg  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

100% Performance Guaranteed

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, MplexRbPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE636
DescriptionAnti-monomethyl Histone H3 (Lys4) Antibody, clone RM140
Alternate Names
  • Histone H3.3
  • Histone H3
Background InformationHistone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
References
Product Information
FormatPurified
PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Key Applications
  • Western Blotting
  • Multiplexing
Application NotesMultiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Biological Information
ImmunogenA linear peptide corresponding to human Histone H3 monomethylated at Lys4.
CloneRM140
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • H3F3A
  • H3.3A
  • H3F3
  • PP781
  • H3F3B
  • H3.3B
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Recombinant HeLa protein and HeLa acid extract.

Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys4) in HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
MABE636 04055977181388

Documentation

Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 Certificados de análisis

CargoNúmero de lote
Anti-monomethyl Histone H3 (Lys4), clone RM140 -QVP1503213 QVP1503213
Anti-monomethyl Histone H3 (Lys4), clone RM140 Monoclonal Antibody VP1705170

Licencias necesarias e Información técnica

Cargo
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.