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07-212 Anti-dimethyl-Histone H3 (Lys9) Antibody

07-212
100 µL  
Purchase on Sigma-Aldrich

Ofertas especiales

Descripción

Replacement Information

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Ch, Vrt, Yeast (S. cerevisiae)ICC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-212
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-dimethyl-Histone H3 (Lys9) Antibody
Alternate Names
  • H3K9me2
  • Histone H3 (di methyl K9)
  • H3 histone family, member T
  • histone 3
  • H3
  • histone cluster 3
  • H3
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
Control
  • HeLa, NIH/3T3 or COS cell extracts.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 5 mg/mL BSA, 0.05% sodium azide before the addition of glycerol to 30%.
Quality LevelMQ100
Applications
ApplicationAnti-dimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9) & has been validated in ICC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesNot recommended for ChIP.
Biological Information
ImmunogenKLH-conjugated synthetic peptide corresponding to amino acids 6-13 (TAR[dimethyl-K]STGG-C) of Histone H3 with C added to the Cterminus for conjugation. The immunizing sequence is conserved from Tetrahymena to human.
Epitopea.a. 6-13
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityMethylated Histone H3 (Lys9). Specificity demonstrated by peptide inhibition assay using methylated and unmethylated peptides. Slight cross-reactivity to the trimethyl Lys27 Histone H3.
IsotypeIgG
Species Reactivity
  • Human
  • Chicken
  • Vertebrates
  • Yeast (S. cerevisiae)
Species Reactivity NoteHuman. Expected to cross react with fission yeast and chicken.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol
  • HIST3H3
  • H3FT
  • MGC126886
  • H3t
  • MGC126888
  • H3T
  • H3/g
  • H3.4
  • H3/t
Modifications
  • Methylation
Purification MethodImmunoaffinity Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 136 amino acids; 15508 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testicular cells.
DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
Molecular Weight17 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3
(cat# 14-411).

Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
07-212 04053252589287

Documentation

Anti-dimethyl-Histone H3 (Lys9) Antibody Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-dimethyl-Histone H3 (Lys9) Antibody Certificados de análisis

CargoNúmero de lote
Anti-dimethyl-Histone H3 (Lys9) - 20449 20449
Anti-dimethyl-Histone H3 (Lys9) - 22704 22704
Anti-dimethyl-Histone H3 (Lys9) - 23032 23032
Anti-dimethyl-Histone H3 (Lys9) - 23424 23424
Anti-dimethyl-Histone H3 (Lys9) - 24837 24837
Anti-dimethyl-Histone H3 (Lys9) - 27271 27271
Anti-dimethyl-Histone H3 (Lys9) - 27563 27563
Anti-dimethyl-Histone H3 (Lys9) - 3234503 3234503
Anti-dimethyl-Histone H3 (Lys9) - 4029282 4029282
Anti-dimethyl-Histone H3 (Lys9) -2763992 2763992

Referencias bibliográficas

Visión general referenciasAplicación EspeciePub Med ID
Hierarchical clustering of breast cancer methylomes revealed differentially methylated and expressed breast cancer genes.
Lin, IH; Chen, DT; Chang, YF; Lee, YL; Su, CH; Cheng, C; Tsai, YC; Ng, SC; Chen, HT; Lee, MC; Chen, HW; Suen, SH; Chen, YC; Liu, TT; Chang, CH; Hsu, MT
PloS one  10  e0118453  2015

Mostrar resumen
25706888 25706888
The preRC protein ORCA organizes heterochromatin by assembling histone H3 lysine 9 methyltransferases on chromatin.
Giri, S; Aggarwal, V; Pontis, J; Shen, Z; Chakraborty, A; Khan, A; Mizzen, C; Prasanth, KV; Ait-Si-Ali, S; Ha, T; Prasanth, SG
eLife  4  2015

Mostrar resumen
25922909 25922909
Alpha7 nicotinic acetylcholine receptor is required for blood-brain barrier injury-related CNS disorders caused by Cryptococcus neoformans and HIV-1 associated comorbidity factors.
Zhang, B; Yu, JY; Liu, LQ; Peng, L; Chi, F; Wu, CH; Jong, A; Wang, SF; Cao, H; Huang, SH
BMC infectious diseases  15  352  2015

Mostrar resumen
26285576 26285576
Methylation of histone H3 lysine 9 occurs during translation.
Rivera, C; Saavedra, F; Alvarez, F; Díaz-Celis, C; Ugalde, V; Li, J; Forné, I; Gurard-Levin, ZA; Almouzni, G; Imhof, A; Loyola, A
Nucleic acids research  43  9097-106  2015

Mostrar resumen
26405197 26405197
The potential of GMP-compliant platelet lysate to induce a permissive state for cardiovascular transdifferentiation in human mediastinal adipose tissue-derived mesenchymal stem cells.
Siciliano, C; Chimenti, I; Bordin, A; Ponti, D; Iudicone, P; Peruzzi, M; Rendina, EA; Calogero, A; Pierelli, L; Ibrahim, M; De Falco, E
BioMed research international  2015  162439  2015

Mostrar resumen
26495284 26495284
Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
PloS one  10  e0134586  2015

Mostrar resumen
26226299 26226299
An H2A histone isotype regulates estrogen receptor target genes by mediating enhancer-promoter-3'-UTR interactions in breast cancer cells.
Su, CH; Tzeng, TY; Cheng, C; Hsu, MT
Nucleic acids research  42  3073-88  2014

Mostrar resumen
24371278 24371278
The interaction of MYC with the trithorax protein ASH2L promotes gene transcription by regulating H3K27 modification.
Ullius, A; Lüscher-Firzlaff, J; Costa, IG; Walsemann, G; Forst, AH; Gusmao, EG; Kapelle, K; Kleine, H; Kremmer, E; Vervoorts, J; Lüscher, B
Nucleic acids research  42  6901-20  2014

Mostrar resumen
24782528 24782528
Epigenetic chromatin modifications in barley after mutagenic treatment.
Braszewska-Zalewska, A; Tylikowska, M; Kwasniewska, J; Szymanowska-Pulka, J
Journal of applied genetics  55  449-56  2014

Mostrar resumen
24939040 24939040
Cerebellar oxidative DNA damage and altered DNA methylation in the BTBR T+tf/J mouse model of autism and similarities with human post mortem cerebellum.
Shpyleva, S; Ivanovsky, S; de Conti, A; Melnyk, S; Tryndyak, V; Beland, FA; James, SJ; Pogribny, IP
PloS one  9  e113712  2014

Mostrar resumen
Western Blotting25423485 25423485

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Life Science Research > Antibodies and Assays > Primary Antibodies