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AB4332 Anti-acetyl-MVH (Lys405) Antibody

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Anti-acetyl-MVH (Lys405) Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Stem Cell Research, Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: Ddx4, Vasa UniProt Number: Q61496
AB4332
100 µL  
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Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, R, PoDB, WB, ICC, PIA, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB4332
DescriptionAnti-acetyl-MVH (Lys405) Antibody
Alternate Names
  • Probable ATP-dependent RNA helicase DDX4
  • DEAD box protein 4
  • Mvh
  • Vasa homolog
Background InformationMVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.
References
Product Information
FormatAffinity Purified
Control
  • Non-acetylated and acetylated MVH (Lys405)
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.
Key Applications
  • Dot Blot
  • Western Blotting
  • Immunocytochemistry
  • Peptide Inhibition Assay
  • Immunoprecipitation
Application NotesWestern Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.
EpitopeAcetylated Lys405
HostRabbit
SpecificityThis antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.
Species Reactivity
  • Mouse
  • Human
  • Rat
  • Pig
Species Reactivity NoteDemonstrated to react with Mouse and Human.
Predicted to react with Rat and Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ddx4
  • Vasa
Modifications
  • Acetylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May play a role in germ cell development. May play a role in sperm motility.

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. N-terminus interacts with RANBP9. Interacts with PIWIL2 and MAEL.
Molecular Weight76 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
AB4332 04053252596537