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ST1704 Anti-TOMM22 Mouse mAb (4G4)

This Anti-TOMM22 Mouse mAb (4G4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM22.

Anti-TOMM22 Mouse mAb (4G4): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Translocase of Outer Mitochondrial Membrane 22

ST1704
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Número de referenciaEmbalaje Cant./Env.
ST1704-100UG 100 μg
Description
OverviewRecognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.
Catalogue NumberST1704
Brand Family Calbiochem®
SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
Application Data
Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
Clone4G4
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
ST1704-100UG 04055977207330

Documentation

Anti-TOMM22 Mouse mAb (4G4) Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Translocase of Outer Mitochondrial Membrane 22
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of rat TOMM22 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse TOMM22 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (1-5 µg/ml). Detection: chemilimunescence.

Detection of human TOMM22 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (10 µg/ml). Detection: fluorescence.

Detection of human TOMM22 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-TOMM22 Mouse mAb (4G4) (Cat. No. ST1704) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.
BackgroundThe protein encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.
HostMouse
ImmunogenFull-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein
Clone4G4
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlPC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue
Negative control293T cells
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review